Microcystest
Microcystest - Imagen 2

Microcystest

Quantify in your water sample the more than 80 variants of microcystins with an simple 30-minutes enzymatic test that also indicates the potential associated toxicity.

 

Do you need to ensure that water does not contain microcystins above the legal limits?
Do you need to know the potential toxicity associated to microcystins?
Are you worried about intracellular microcystins released during water purification and you need a reliable method for their extraction?

 

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HOW DOES IT HELP YOU?

 

Microcystest reproduces the biological action of microcystins, detecting all variants independently of their chemical structure. Therefore, the quantitative result obtained with Microcystest will reflect the potential toxicity associated to the water sample.
Microcystest can be used analysis of both raw water and treated water in drinking water stations. The tests can identify free and intracellular toxins. You will be able to know the toxicity associated with microcystins and nodularins in up to 43 samples per assay and just using basic lab equipment and in just 30 minutes.

MATRIX
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APPLICATIONS AND USERS
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ASSAY PROCEDURE

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1. Apply Samples/Standard:

+ Phosphatase

+ Substrate.

2. Incubation:

37ºC/30 min.

3. Results:

Photometrical reading at 450 nm

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Nombre del video

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1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugado:

Incubación

3. Results:

Lectura fotométrica a 450nm

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TEST PRINCIPLE

Microcystest is based on the inhibition of the enzymatic activity of PP2A by microcystins, reproducing the mechanism of action of these toxins in human beings. Therefore, it detects and quantifies the potential toxicity of a sample derived from microcystins and nodularins. Under normal conditions the phophatase is able to hydrolyse a specific substrate that can be detected at 405 nm. Samples containing microcystins will inhibit the enzyme activity proportionally to the amount of toxin contained in the sample.

TECHNICAL DATA

  • Assay time: 30 minutes
  • Range of quantification: 0.25-2.5 µg/L
  • Formats: plate (48 or 96 wells) and tube

 

Validations:

Internal validation following Eurachem guide.

Assay verified within “Environmental Technology Verification Program” under the cooperation of the Environmental Protection Agency USA.

Participation in the intercollaboration study Labaqua-Calitax (circuito ielab)

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