ELISA Pig CRP

ELISA Pig CRP

Detect problems of health and poor welfare in your animals with high sensitivity testing C-reactive protein. Acuvet ELISA pig CRP is a species-specific, sensitive and reliable test to determine this biomarker using basic equipment (ELISA reader).

 

Do you want an indicator of animal health and welfare status that is objective and easy to determine?
Do you want to detect subclinical infections or evaluate if the treatments applied to the animals are affective?
Do you want to measure pig CRP in a study with all the guaranties?
Do you need a sensitive and reliable method?

 

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HOW DOES IT HELP YOU?

 

CRP is a sensitive and fast responder biomarker to assess the animal health and welfare status or quantify inflammation in a trial. Used in combination with Pig-MAP provides high sensitivity in the detection of all kind of infectious and inflammatory diseases on the farm. The Acuvet ELISA pig CRP method is pig-specific and has been properly validated. It provides higher sensitivity than the turbidimetric method and is equally valid for hemolyzed samples.

MATRIX
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APPLICATIONS AND USERS
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ASSAY PROCEDURE

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1. Sample:

Dilute sample/pattern and apply

Room Temperature Incubation

2. Conjugate:

Wash and apply conjugated.

Incubation at room temperature.

Wash and add substrate.

3. Result:

Photometrical reading at 450nm

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Nombre del video

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1. Dilución de la muestra:

Aplicar muestra/patrón. Incubación.

2. Conjugate:

Incubation

3. Results:

Photometrical reading at 450nm

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TEST PRINCIPLE

Acuvet ELISA pig CRP is a sandwich-type ELISA, based on polyclonal antibodies specific for pig CRP. The assay is calibrated with a swine-specific internal reference material, as no international standard is available for pig CRP.

TECHNICAL DATA

  • Specific for swine
  • Limit of detection: 2 ng/mL
  • Working range: 7-220 ng/mL
  • Assay time : 90 minutes

 

Validations:

Internal validation according to Eurachem´s guidelines

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